The growth performance, cost, haematological and serum parameters, egg production, and egg quality traits of layers offered cassava root meal (CRM) diets supplemented with or without activated charcoal were examined in a 14-week feeding trial. A total of 256 ISA Brown point of lay birds (18 weeks old) were divided into 8 dietary treatments in a completely randomized design. Each treatment was replicated 4 times with 8 birds per replicate in a 2x4 factorial arrangement. A maize-wheat offal-concentrate diet was the control diet. The maize was replaced with CRM at 25, 50, and 75% for activated and non-activated charcoal diets. The values obtained for final live weight and weight change, average daily feed intake, hen-day production, egg weight, and feed conversion ratio, all differed significantly (p0.05) affected by activated charcoal supplementation. However, albumin, Aspartate aminotransferase and triglyceride were significantly (p<0.05) affected. It was concluded that replacement of maize with CRM with or without activated charcoal supplementation beyond 50% level reduced the feed intake and hen day production of layers, and charcoal supplementation at 40g/kg feed in cassava-based diets gave a better production performance and improved health status of layers.