Background: A second-generation platelet concentrate called platelet-rich fibrin (PRF) has been the subject of extensive research for the past two decades. The debate over relative centrifugal force (RCF), revolutions per minute (RPM), and relative time in the production process of various platelet concentrates has significantly complicated the topic during the last few years. The following factors need to be taken into account in order to produce the precise sub-type of PRF: Rotor angulation for the tube holder, Revolutions per minute (RPM), Relative centrifugal force (RCF), Rotor dimensions, Centrifugation model employed, Composition, size & form of used tubes. Objective: The study's objective was to assess how these variables interacted to produce the three different subtypes of PRF (S-PRF, A-PRF, and I-PRF) solely by adjusting the RPM and Duration without altering the rotor's angulation or size. Materials and Methods: Fifteen healthy volunteered patients for the study underwent surgical removal of impacted mandibular 3rd molars. Their peripheral blood was drawn and three subtypes of PRF were produced using a tabletop centrifuge (REMI C-852). Conical end glass tube with rubber stopper & counterweight balance were used. S-PRF, A-PRF, and I-PRF were obtained at 2700 rpm for 12 minutes, 1500 rpm for 14 minutes, and 700 rpm for 3 minutes, respectively. Statistical analysis was done using independent t-test in IBM SPSS 26.0 software. Results and Conclusions: The three subtypes of PRF have their own indications in different fields of oral and maxillofacial surgeries. They have enriched growth factors for excellent wound healing & tissue regeneration.