Background: Effective nursing handoff communication is critical to delivering safe, high-quality patient care. Aim: This study aimed to assess the effect of nursing handoff educational bundle on nurse interns' knowledge sharing. Research design: A quasi experimental one group pre- posttest design used to conduct this study. Settings: The study was conducted at Kafr El-Sheikh University Hospital .Subjects: Included (95) nurse interns enrolled in internship training year. Tools: the data for this study were collected through three tools, namely handoff knowledge questionnaire" handoff communication competence observational checklist and knowledge sharing questionnaire. Results: Nurse Interns age ranged between 22 to 24years .73.7% of them were female, the majority of them (73.7%) had secondary school, slightly less than two thirds (64.2%) of them had no previous work experiences. About one third (30.5%) of nurse Interns' had satisfactory knowledge regarding nursing handoff before the intervention,This increased to (91.6%) post intervention and slightly declined to (87.4%) in follow up phase. Regarding nurse interns' handoff communication competence about (8.4%) had high competence in pre-intervention phase which increased to (92.6%) in post intervention and slightly declined to (68.4%) in follow up phase. Considering nurse interns' knowledge sharing about(83.2%) had satisfactory knowledge which increased to(95.8%)|post intervention and slightly declined to(94.7%)in follow up phase .Conclusion: The implementation of nursing handoff educational bundle was effective in improving nurse intern's handoff knowledge and communication competence as well as knowledge sharing. Recommendation: Regular workshops and seminars for nurse interns should be held periodically and incorporating nursing handoff and knowledge sharing in undergraduate curriculum to increase competence as well as knowledge sharing.