Manuscript Title:

BIOREMEDIATION OF HEAVY METALS FROM WASTEWATER THROUGH SOIL BACTERIA

Author:

ALIYA KHALID, YAHYA KHAN, RANA HADI, SAYYADA GHUFRANA NADEEM, SANA AHMAD, ATEEQ AHMAD AFRIDI, Dr. MAJID HUSSIAN

DOI Number:

DOI:10.17605/OSF.IO/CV3MU

Published : 2023-04-10

About the author(s)

1. ALIYA KHALID - Department of Microbiology, Abdul Wali Khan University Mardan, KP, Pakistan.
2. YAHYA KHAN - Department of Agriculture and Veterinary Medicine, University of Padua, Italy.
3. RANA HADI - Department of Zoology, Jinnah University for Women, Karachi, Sindh, Pakistan.
4. SAYYADA GHUFRANA NADEEM - Department of Microbiology, Jinnah University for Women, Karachi, Sindh, Pakistan.
5. SANA AHMAD - Department of Biochemistry, Bahria University Medical and Dental College, Karachi, Sindh, Pakistan.
6. ATEEQ AHMAD AFRIDI - Department of Microbiology, Abdul Wali Khan University Mardan, KP, Pakistan.
7. Dr. MAJID HUSSIAN - Department of Forestry and Wildlife Management, University of Haripur, KP, Pakistan.

Full Text : PDF

Abstract

Currently, water contamination is one of the leading problems worldwide. Unprocessed industrial waste removal into the environment creates a serious problem for freshwater, marine living organisms, and human beings. Among pollution-causing industries, the textile industry achieves major attention from environmentalists due to the expenditure of a large volume of wastewater and chemicals during various manufacturing processes. Wastewater contains carcinogenic organic and inorganic materials such as heavy metals. Nearly all the physicochemical procedures do not provide essential parameters for removing heavy metals from industrial wastewater. Bioremediation is a successful way the removal of heavy metals from wastewater. Most of the bacteria are resistant to heavy metals and are used as a potential source of bioremediation of heavy metals. In this research, four bacterial species were isolated from soil samples of District Mardan KP, Pakistan. Morphological, physiological, and biochemical characteristics of these isolates were observed. Wastewater samples were collected from Sugar Mill and Marble industries located at Nowshera road District Mardan KP, Pakistan. After that wastewater containing heavy metals was analysed by Atomic Absorption Spectrometry and three metals, Cadmium, Chromium, and Iron was identified. After that four-isolated bacterial species (Bacillus subtilis, Acinetobacterboumannii, Pseudomonas aeruginosa, and Lactobacillus acidophilus) were used to treat the heavy metals in industrial wastewater. After the successful incubation period of 5 days, among these four isolates, three bacterial isolates successfully eliminated the heavy metals from the industrially contaminated water. The present study shows that heavy metal tolerant bacterial species effectively degrade the heavy metals from industrial wastewater.


Keywords

Bioremediation, Heavy Metals, Wastewater, Soil Bacteria, Pakistan.