Background: Glaucoma is a common condition which affects people worldwide. It is a group of eye diseases characterized by elevated intraocular pressure (IOP) that causes damage to the optic nerve, which can lead to progressive loss of vision. Awareness and knowledge related to the nature of glaucoma and treatment regimen is the first step in planning for disease management. Aim: The aim was to evaluate the effect of proposed nursing care protocol on selected outcomes among patients with glaucoma. Design: Quasi-experimental pre-posttests nonequivalent control group design was used in the study. Setting: The study was conducted in the Glaucoma Outpatient Clinic, in one of university hospital in Cairo, Egypt. Sample: A purposive sample of 62 adult male and female patients confirmed diagnosis with primary open angle glaucoma in both eyes over period of six weeks. Tools: five tools were utilized to collect data; Personal and Background Information Form; Pre-Post Patients' Knowledge Assessment Test; Glaucoma Manifestations Assessment Tool; Glaucoma Activity Limitation-9 Questionnaire and Patients’ Practices Checklist Regarding Eye Care. Results: The mean age of patients under the study was 55.4±5.1 and 52.8±4.6 in the study and control group respectively. A highly significant differences were found between study and control group (p>0.05) in relation to outcome variables (glaucoma knowledge, activity limitation, and practices of eye care). Conclusion: the scores of glaucoma knowledge, activity limitation, and practices of eye care were higher after implementation of nursing care protocol than the scores of the control group who received routine hospital care. Recommendations: Application of nursing protocol of care in glaucoma outpatient clinic and guide the nurses to motivate the patients with glaucoma to follow the guided information.