The present research work was done at NARC Islamabad, to search the potential of combine organic application, bio fertilizers & chemicals for improving micronutrient concentration in spinach. Different treatments were designed to compare with NP fertilizer, bio fertilizers and Organic fertilizer to observe micronutrient concentration in shoot and root at various stages of development & growth. Highest concentration of iron shoot was noted with isolates Azorhizobium, Rhizobium and PSM-Q. At different stages of growth & development. Maximum content of root Iron was noted with Azorhizobium and Rhizobium. Among the isolates, Azorhizobium, Rhizobium and PSM-QA performed relatively better in enhancing zinc content in spinach shoot at all the three stages of growth. Highest root zinc concentration was recorded with inoculum Azorhizobium and PSM-QA. The results suggested that bacterial inoculation significantly increased the micronutrient content of spinach as compared with the application of chemical fertilizers, organic fertilizer and the control. We communicate sound proofs to recommend bacterial inoculation as a suitable and most reliable substitute of chemical fertilizers that promote/enhance the growth of spinach along with improving the rhizosphere for field crop production. As a result, it may be argued that microbial inoculation enhanced efficient nutrient uptake, resulting in higher-quality plants.