Background: Work is considered a basic part of one's life experience. Every type of work brings with its’ risks and health hazards. Aim of study: This Study aimed at evaluating the effect of implementing occupational hazards training on the knowledge and practice related to safety measures among staff nurses. Subject and method: this study was conducted at Egypt air hospital for Research and treatment. It is affiliated to Ministry of Civil aviation using a quasi-experimental one group pre- posttest design on 185 staff nurses. Data were collected by using two tools namely, self -administered knowledge questionnaire and observation checklist for staff nurses' practice regarding safety measures. Results: revealed, that 31% of studied staff nurses had satisfactory of total knowledge at pre intervention phase, So, there was highly statistically significant improvement in staff nurses’ total knowledge in the phases of post 71% and the follows up80%, total practice was low at pre- intervention phase, slightly more than 51% of studied nurses had adequate total practice. So, there was highly statistically significant improvement in staff nurses in all areas and total practice in the post 88% and follows up phases 89%, there a highly statistically significant moderate positive correlation between all knowledge categories', that there was highly statistically significant moderate positive correlation between all practice areas, additionally there was a statistically significant weak positive correlation between knowledge and practice. Conclusion: The study concluded that there was statistical significant improvement in nurses' knowledge and practice after implementation of the program. Also there was weak positive correlation between nurse's knowledge and Practice. Recommendation: Enhancing knowledge and skills of safety measures through the orientation for newly staff nurses as well as, continuous in-services training. And hospital management must establish safe environment through adequate infrastructure, equipment, and facilities.