Medicinal plants are the main source of bioactive compounds which are used for treatment of fetal and infectious diseases. In recent era it is also increasing the demand of medicinal plants due to the presence of important drugs. Persicaria hydropiper and Caralluma fimbriata are the source of the active compounds used as traditional medicines. P. hydropiper belong to family Polygonaceae and C. fimbriata to Apocynaceae. Cancer is a syndrome in which uncontrol division of cells are present in normal body tissues. It is spread by blood and lymphatic system to other body parts. During the present study the anticancer potential of different extracts of P. hydropiper and C. fimbriata against breast cancer cell lines (MCF-7) was evaluated. Breast cancer cell lines were seeded in 96 well plates and treated with different extracts of P. hydropiper and C. fimbriata. The MTT(3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide) dye was added after incubation of 24 hours. Then check the absorbance by plate reader at 570 nm wavelength. The IC50 value of aqueous extracts of C. fimbriate was 5.07 μg/ml while chloroform extracts of P. hydropiper showed excellent anticancer activity against the breast cancer cell line (MCF-7) with an IC50 value of 1.23μg/mL. The availability of plant extract also contributes to the treatment of cancer in developing countries. P. hydropiper n-hexane extract and C. fimbriata chloroform and aqueous extracts possess very potent anticancer activity against MCF-7 cell lines.