Helicobacter pylori (H. pylori) is a gram negative, micro aerophilic bacterium that can inhabit various areas of stomach, particularly the antrum. It causes a chronic low-level inflammation of the stomach lining and is strongly linked to the development of duodenal and gastric ulcers and stomach cancer. The present study was carried out from June 2017 to July 2018 to investigate the prevalence of H. pylori infection in rural and urban areas of District Peshawar. During this period blood samples along with history were collected from 160 individuals of rural and urban areas of District Peshawar. Questionnaires were used as a tool for data collection. It was observed that apparently 55% of healthy persons were harboring the H. pylori antibodies and the gender wise prevalence was 31.25% in males and 23.75% in females. During this study, a high prevalence rate (38.12%) was found in the individuals belonging to urban areas as compared to peoples of rural areas (16.87%). Age wise prevalence was also observed, and the highest prevalence rate (21.25%) was found in (21-30) years of age. A higher prevalence rate was found in drivers (26.25%), followed by students (8.75%). The socioeconomic status wise prevalence of H. pylori shows a higher prevalence rate (35%) of infection in individuals belonging to lower class families as compared to other classes. During this study, it was observed that those people who used to eat in hotels were more infected (36.25%) as compared to those who used to eat in homes (18.75%) respectively.