Serum proteins have a variety of uses, including immune system activity and lipid, vitamin, hormone, and mineral transfer in the circulatory system. Although serum proteins are present in very high quantities, their composition is unevenly distributed. This means that only 22 proteins, including serum globulins, albumin, and fibrinogen, make up 99% of all serum proteins, with the remaining 1% of blood proteins being made up of circulatory proteins with low abundance as well as proteins released by apoptotic, necrotic, and liver cells. Except for gamma globulins, which are made by the immune system, the liver, and bowels are responsible for secreting the majority of blood proteins. In the current research, we sought to assess the serum samples obtained from patients with gastric cancer and compare them to serum samples from healthy individuals. The serum samples were estimated using the Bradford method and performed SDS PAGE analysis. In light of protein-based studies using serum samples, this paper emphasizes the significance of serum sample collection, handling, and storage.