This study was performed to evaluate the resistance rates to antibiotics among bacteria isolated from urine culture from Syrian female’s patients from different age categories. The most common uropathogen identified in urine isolates was E. coli. 2% of bacteria isolates were resistant to one antibiotic, while 98% bacteria isolates were resistant to at least 2 antibiotics. An extremely high in-vitro resistance rate (more than 90%) was remarked among bacteria isolates, especially toward Cefotaxime, Nalidixic acid, Cefexime, Cephalexin and Cefpodoxime. For Cefetriaxone, Cefuroxime, and Trimethoprim–Sulfamethoxazole, resistance rates ranged between 70%-80%. Resistance rates for Nitrofurantoin, Amoxicillin - Clavulanic acid, Ciprofloxacin and Norofloxacin were between 37% and 60%. For some antibiotics, resistance rates were high from early life and continue with nearly the same values along different age stages (Trimethoprim–Sulfamethoxazole, Cephalexin and Cefexime), for other, resistance rates progressively increased with increasing age of patients (Nitrofurantoin, Ciprofloxacin and Norfloxacin). Finally, Amikacin, Gentamycin, and Imipenem showed good sensitivity (more than 90%) in almost all age’s groups.