The field experiments were carried out to evaluate the impacts of rice rotation on rice productivity and soil properties. The study was conducted with the aim of evaluating the impact of rice rotation with upland crops in improving soil quality as well as the growth and yield of rice crop 2022-2023 on the alluvial soil cultivation in Tri Ton district, An Giang province. The field experiment was arranged in a completely randomized block design (RCBD) with 3 treatments and 4 replications. Three plant types of rotations were evaluated: ricerice-rice (control-RRR), rice-soybean-rice (RSOR), rice-sesame-rice (RSER). The collected data for evaluation include soil physical properties such as bulk density, porosity, and available water holding capacity. Soil chemical properties such as pH, EC, available nitrogen, available phosphorus, exchangeable K, soil organic matter (SOM); biomass and rice yield. For the soil chemical parameters, the RSOR treatment showed an effective improvement in the available N, available P, exchangeable K and OM in the soil. Rice yield and rice biomass in the end crop tended to increase in the rotation treatments with soybean and sesame compared to the 3-crop intensive rice cultivation. Long-term experiments need to be continued to better see the effect of crop rotation on soil fertility (especially soil physical fertility), as well as crop growth and yield.