During the present study, wheat field of of Larkana (district) having four talukas namely Larkana, RatoDero, Dokri and Bakarani were surveyed during the first week of November 2020 to April 2021. Total 1623 specimens were collected from the wheat fields that were differentiated into four families such as Araneidae, Tetragnathida, Oxyopidae, and Lycosidae. All families were researched out up to Genera and species level such as Argiope trifasciata, A. pradhani, Tetragnatha Javana, Lycosa terrestris, Pardosa birmanica, Oxyopes marginalis. All families, all genera as well as up to species are first time recorded from different area of district Larkana. Species of above families up to generic level and species levels which were identified with help of taxonomical keys. The maximum record and dominant family was Araneidae (2- species) trailed by Lycosidae (2-species), Tetragnathidae (01-species) and Oxyopidae (01-species). It proved that 38.44% of the total collections were observed in taluka Larkana followed by taluka Dokri (30.93%), taluka Bakrani (18.22%) and taluka Ratodero (12.07%). This research work will provide basic knowledge about wheat spider’s fauna, mainly in the field of IPM.