Antibiotics were commonly employed as food supplements and growth enhancers in broilers, but they were also linked to harmful side effects. As a result, an alternate source to substitute antibiotic growth enhancers was necessary; such a product is Phyto-biotics, specifically Mentha piperita leaves extract. The goal of the current study was to determine how different doses of M. piperita leaves extract would affect broiler production and E. coli resistance. 100 milliliters of distilled water were used to dissolve 3.8 grams of Mueller hinton agar powder to prepare the culture medium for the invitro experiment. The disc diffusion technique was used to assess the extract's efficacy. For the in-vivo experiment, six major groups made up of 108 oneday-old broiler chicks were assigned at random. The same management and environmental standards applied to all groups. The results showed significant antibacterial activity of the M. piperita extract against E. Coli. An inhibitory zone of 18.67 mm is created by the extract. The results showed that the No Treatment, Only Inoculation group had the lowest total body weight (515.66g/chicken) while the pre-inoculation 2000ppm group had the highest total body weight (930.22g/chicken). The culture of liver and spleen blood on Mueller-Hinton agar revealed no infection in the broilers who were getting treatment. In conclusion, M. Piperita significantly inhibits the growth of E. Coli.