Background: Patient safety is a crucial principle in healthcare, requiring strategic planning, consistent practices, and targeted training programs. Aim: The study examined the impact of patient safety training programs on nursing staff compliance with safety goals and safety culture. Design: A quasi-experimental research was utilized. Setting: The study was conducted at Al Kaser ElAini Teaching Hospital, affiliated with Cairo University. Subjects: All nursing staff (n=40) at the selected critical care units. Tools: Data was collected via a patient safety knowledge test, a safety culture survey, and an observational checklist of compliance with safety goals. Results: The study revealed significant statistical differences in total knowledge and knowledge dimensions during assessment periods. It also revealed significant differences in total patient safety culture and all dimensions. The total mean score across all dimensions increased significantly from x̄= 65.53 pre-program to x̄=153.79 post-program and x̄=147.00 at follow-up. Additionally, there were significant differences in total compliance with patient safety goals mean scores and all dimensions, with the overall total mean compliance score increased from (x̄=34.64) pre-program to (x̄=56.83) post-program. Conclusion: The study found a significant improvement in nursing staff's knowledge test scores, patient safety culture, and compliance with safety goals immediately and three months after program implementation compared to before. Recommendations: Healthcare administrators should consider implementing regular patient safety training programs to enhance nursing staff knowledge
of patient safety, foster a patient safety culture, and promote compliance with safety goals.