This study employed SDS-PAGE to analyze the impact of the organophosphate insecticide Malathion on the protein profiles of the freshwater fish Channa punctatus across a variety of tissues, including muscle and brain. The findings revealed that there are 10 protein bands responsible for regulating brain functions and 8 responsible for regulating muscle functions. Malathion, at a sublethal dose of 2% Organophosphate, was applied to Channa punctatus throughout the course of many time periods. The study's results show a decrease in the bands' frequency and intensity over time, as well as the formation of certain new pesticide- opposing protein bands. Pesticide's impact on protein concentration in skeletal muscle and brain tissue, as detected by 7.5% SDS-PAGE. Standard marker proteins were used to determine protein banding patterns, and Rm values were then determined. Electrophoretograms of both skeletal muscle and brain from the present investigation displayed protein band heterogeneity with several variations.