Objective: The study aimed to measure the transient axial length (AXL) changes after short-term exposure to blue light before and after hyperopic defocus. Methodology: An interventional study design (quasi- experimental pre and post-interventional design) was conducted at Al-Shifa Trust Eye Hospital among

optometry students using a non-probability judgmental sampling technique. Visual acuity was measured with LogMAR, and refractive status was determined with a Retinoscope. Blue light exposure was administered using a Blue Laser Pointer emitting a 405nm wavelength. Hyperopic defocus was induced using a -3.00DS lens in a trial frame. The IOL Master Zeiss 700 was used to measure the AXL. Data were entered and analyzed through online web-based software, Data tab. Results: A total of 30 subjects, including 06 (20%) males and 24 (80%) females, with a mean age of 20.67 ± 0.96, participated in the study. Comparing Pre-defocus Axial length (PDAXL) and After Defocus Axial length (ADAXL) following 30 seconds (23.2 ± 0.69, 23.19 ± 0.68, p 0.006), 60 seconds (23.2 ± 0.68, 23.2 ± 0.68, p 0.056), and 90 seconds (23.2 ± 0.69, 23.2 ± 0.69, p 0.002) of exposure to blue light, a statistically significant difference was observed among the 30-second and 90-second exposure groups (p 0.006 and p 0.002, respectively), but not among the 60-second group (p 0.056). Conclusion: The study concludes that short-wavelength exposure and hyperopic defocus together cause axial length shortening, which could be employed to manage myopia progression.