The most prevalent thyroid gland condition is hypothyroidism which occurs when the thyroid's follicular cells are unable to produce enough thyroid hormones (THs) needed for body to function normally. Tetraiodothyronine (thyroxine), T4 and tri-iodothyronine (T3) are the two fundamental hormones produced by the thyroid gland (TG).THs are basically responsible for controlling the body’s metabolism. Iodine is a major contributor to the global rise in thyroid-related illnesses. Additionally, many clinical manifestations, indications, and symptoms that the patients showed were examined, and the condition was established in relation to those symptoms. This cross sectional study has been quantitatively carried out and depends on a method of statistical data analysis. This cross-sectional study was carried out over a period of one year from July 2019 to November 2020. This case study conducted at two famous hospitals of Jamshoro namely LUMHS and NIMRA. Total participants were 105. Sampling was random; samples were males, females and children. The study has been conducted to analyze the hypothyroid patients coming from various parts of Sindh at OPDs of NIMRA and LUMHS who have been severely affected with other disorders as well. The study involved special designed questionnaire and laboratory test reports for the purpose of analyzing the pure patients of hypothyroidism. Thyroid function test (TFT) was done using enzyme-linked immunoassay (ELISA) techniques, Radioimmunoassay (RIA) test kits.