Objective: To find out prevalence of knee pain in female footballers of twin cities i.e., Rawalpindi and Islamabad. Methods: Cross-sectional study from March 2022 to June 2022, was carried out at multiple football clubs in Rawalpindi and Islamabad. Sample comprised 249 female football players aged between 10 and 25 years. A non-probability convenience sampling method was employed. Data collection was carried out using a self-structured close-ended questionnaire. Data analysis was performed using SPSS version 25. To explore association between knee pain and dominant leg, chi-square test was utilized. Results: Findings presented estimates of knee pain prevalence across different time frames. Point prevalence of knee pain was reported at 29%, while rates for past month, three months, six months, and one year were 45%, 57%, 65%, and 73% respectively. Moreover, lifetime prevalence of knee pain among participants was found to be 82%. Among participants, 82% had a dominant right leg, while 18% had a dominant left leg. Of those experiencing knee pain, 58% reported pain in right knee, while 23% reported pain in left knee. These findings demonstrate a significant correlation between knee pain and the dominant side of the body (p < 0.01). Conclusion: Study's findings confirmed a high prevalence of knee pain among female footballers, with a noteworthy association between knee pain and dominant leg.