Objective: The aim of this study was to evaluate the Child-Pugh score distribution among a group of patients with Chronic Liver Disease (CLD) and to measure their hepatic enzyme levels. Materials and Methods: This descriptive cross-sectional study was conducted at Jinnah Postgraduate Medical Center in Karachi, Pakistan. We included 200 participants based on specific inclusion and exclusion criteria. These subjects were categorized into four groups: a control group and groups A, B, and C based on their ChildPugh scores. All participants provided written informed consent. Data were recorded and analyzed using SPSS Version 23. Results: There were notable differences among the groups in terms of Total Serum Bilirubin, Albumin, INR, Alkaline Phosphatase (ALP), Aspartate Aminotransferase (AST), and Alanine Aminotransferase (ALT). It was observed that Total Serum Bilirubin and INR values rose, whereas Albumin levels fell as liver disease progressed. The concentrations of ALP, AST, and ALT also escalated with increasing Child-Pugh scores. Conclusion: This investigation highlighted significant disparities in hepatic biochemical indices among the control and various Child-Pugh score groups, underscoring the influence of liver disease severity on these indicators. This knowledge enhances our understanding of the biochemical landscape in CLD patients and supports the improvement of clinical evaluation and management approaches for individuals at different stages of liver disease.