Eucalyptus is attacked by various fungal and bacterial pathogens that have caused huge economic losses globally. Leaf spot disease of Eucalyptus camaldulensis caused by Curvularia lunata was reported in Shorkot Irrigated Forest Plantation Punjab, Pakistan. In the current study, various Trichoderma spp. i.e., T. viride, T. harzianum, T. Koningii, T. Asperellum, and T. Virens were evaluated during invitro. Invivo studies applied (1x105 , 1x106 , 1x107 conidia/mL of water) to manage the leaf spot of Eucalyptus camaldulensis caused by Curvularia lunata in the Research Area, Department of Forestry and Range Management, University of Agriculture, Faisalabad (UAF), Pakistan during (2021-22). The findings revealed that highest fungal growth was inhibited during invitro study by Trichoderma viride (16.693 mm) followed by Trichoderma harzianum (20.737mm), Trichoderma Koningii (24.467mm), Trichoderma Virens (24.841mm), Trichoderma Asperellum (24.841mm) as compared to control (32.246mm) respectively. While, the least disease incidence (17.968%) was observed with the combined application of T. viride+ T. harzianum followed by T. viride (27.221%), T. harzianum (31.374%) as compared to control (43.663%) in the greenhouse experiment. The minimum disease incidence (18.134%) was observed with the combined application of T. viride+ T. harzianum followed by T. viride (18.351%), T. harzianum (25.294%) when compared with control (36.885%) respectively when applied in the field experiment. The data were analyzed by using the statistical software Minitab-2018 and interpreted accordingly.