Introduction: Chronic stress significantly impacts physiological and neurological health, causing hormonal imbalances and potentially contributing to neurodegenerative conditions. This study investigates the effects of Moringa oleifera (MO) leaves, recognized for their anti-diabetic and neuroprotective properties, on stressinduced cognitive and behavioral changes in an animal model. Materials and Methods: Rats were divided into five groups: control, stressed, positive control (standard treatment), Moringa-treated healthy rats (MTHR), and Moringa-treated stressed rats (MTSR). Over a 3-week treatment period, behavioral assessments and neurochemical analyses were conducted. Statistical analysis was performed to determine significance. Results: Stress altered acetylcholine (Ach) levels in the brain, indicating cholinergic dysfunction in stress-related behaviors. MO leaf extract supplementation normalized oxidative stress markers and Ach levels in both stressed and healthy rats. This normalization was associated with reduced anxiety and depression in MTSR and improved memory function. Chronic stress significantly increased anxiety, depression, and memory impairment in rats, accompanied by elevated malondialdehyde (MDA) levels and reduced activities of antioxidant enzymes (superoxide dismutase, catalase, glutathione). Conclusion: These findings highlight the therapeutic potential of MO leaves in mitigating stress-induced behavioral and neurochemical disruptions. By modulating acetylcholine levels, reducing oxidative stress, and enhancing memory function, MO supplementation shows promise for alleviating stress-related symptoms and preventing associated health complications. Further research in human trials is necessary to refine these insights and optimize clinical applications.