The objective of this study was the isolation and characterization of pigmented bacteria, with a focus on exploring their pigment-producing capacities and assessing the practical applicability of these pigments in textile dyeing procedures. Four distinct pigmented bacterial strains—Chryseobacterium meningosepticum (Pf5), Proteus vulgaris (HC), Aeromonas hydrophila (Pf4), Halomonas sp. (AST)—were carefully isolated and subjected to an investigation into optimized culture and media variables. The identified optimal conditions for both growth and pigment production included the use of peptone broth media at 37°C, pH=7, 2-4% salination in the growth media, under shaking and dark conditions. The biopigments were subsequently extracted in organic solvents, including diethyl ether, chloroform, ethanol, and methanol. UV/Vis spectrophotometry revealed peak absorbance at 440-450nm for pigments extracted using diethyl ether and chloroform. The antibacterial activity assessment confirmed the presence of antibacterial substances within these biopigments. Furthermore, the extracted pigments underwent comprehensive testing for their coloring potential on diverse materials such as fabrics, soap, cotton, and tissue paper, demonstrating a non-degradable colorant. These findings highlight the potential applications of these bacteria in advancing sustainable and environmentally friendly dyeing processes within the textile industry.