Introduction: Lycium Barbarum is a Chinese herbal drug belongs to the Solanaceae family. Lycium Barbarum juice help with a variety of conditions, including cardiovascular effects such as blood pressure and pulse rate, neurological and psychological impacts, musculoskeletal issues and beneficial to increase the life span and it also lowers the risk of arteriosclerosis and arterial hypertension. Aim: The aim of the present study was to examine the effect of Goji Berry (Lycium Barbarum) and high fat diet on Liver Structure and Function tests Total Bilirubin, Bilirubin Direct, Bilirubin indirect, ALT/SGPT, enzymes test AST/SGOT, Alkaline phosphate, GGT, and total protein in different groups of Rabbits to check. Methodology: Rabbits were categorized into 4 groups. Group 1 (Control group): Rabbits of group 1 were treated with normal feed with no dose. Group 2 (Experimental group 1) (Oil + Feed) Rabbits of group 2 were treated with 2ml of oil along with normal feed including grass and bread. Group 3 (Experimental group 2) (Lycium Barbarum + Oil + Normal feed) Group 3 rabbits were fed with 2ml of oil, 4ml of Lycium Barbarum juice and normal feed. The dose was given orally with the help of syringe pipes. Results: parameters of total, direct, and indirect bilirubin, no statistically significant differences were found. The characteristics of total protein, albumin, globulins, alkaline phosphate, and GGT rates were shown to differ significantly. Conclusion: Lycium Barbarum is the best and most affordable herb to treat a variety of conditions affecting both humans and animals, such as infertility, chronic and acute liver illnesses, ALDL, and NADNT disease.