The traditional plants found throughout the world contain many medicinally important constituents but these need to be investigated for their phytochemistry, biological and pharmacological activities. In Pakistan many plants are found which are believed to be medicinally important. In the current study, Convolvolus arvensis Linn whole plant was investigated for their pharmacognostic analysis and biological activities. Quantitative pharmacognostic analysis showed that the extract contains total ash value of 10.4% and moisture content 1.04%. Organoleptic characteristics, macroscopic and anatomical studies were performed. The antifungal activity was evaluated employing five fungal pores and the result revealed that this plant has strongest antifungal activity against Candida albicans and lowest against Aspergillus fumigatus. The highest percentage inhibition of α-amylase for Convolvulus arvensis Linn was shown by the ethanolic extract i.e. 35.43% which shows that the plant is antidiabetic. The current study suggests that the plant has different phytochemical and biological activities but selection of solvent system is the key to explore comprehensive biological actions of plants and isolation of commercially potential compounds.