Smoking is a worldwide problem and it affects both actively and passively. It affects the health of human being as well as animals. The smoking leads to the disturb whole system of the body and harms each organ of body. Tobacco smoke is expected to lead to heart failure and cardiomyopathy. Smoking has a direct impact on cardiovascular system. The objective of the current research study to evaluate effect of tobacco (Nicotiana tabacum) smoke on level of lipid profile in chickens and to determine the histopathological changes in heart tissue of chickens due to exposure of tobacco (Nicotiana tabacum) smoke. A set of 15 chickens were used for this research at the age of the chickens 16 weeks to 18 weeks. Chicken was divided in to three groups into control group, experimental group one and two. Cigarette were purchased from the market and given to the chickens in the form of tobacco smoke for 30 days. Passive smoking exposure technique was used. It was concluded that the lipid profile of experimental group one and two disturbed, the concentration of bad cholesterol e.g., LDL, total cholesterol, VLDL and triglycerides increased while the concentration of good cholesterol HDL decreased due to the passive smoking exposure. The histopathology of heart tissues of control group remained normal, no tissue changes were seen and cardiac muscles were normal, while the heart tissue of showed the mild congestion, shrinkage and atrophy of cardiac muscle fibers was seen and also mild congestion was seen in the myocardium.