The research aims to reveal the impact of the Jordanian constitutional amendments on the principle of separation of powers, compared with several Arab constitutional systems. The importance of the study stems from the importance of the principle of separation of powers, the importance of identifying the extent of its application in the Jordanian constitution, and the extent of the impact of the constitutional amendments of 2011 on it, in addition to the importance of identifying the extent of its application in several Arab constitutions. The researcher relied on the comparative analytical approach in terms of reading the Jordanian constitution and its amendments in force, clarifying the principle of separation of powers in it, and comparing it with the texts of the constitutions of some Arab countries. The research is divided into three comprehensive sections in addition to the introduction and a conclusion containing the most prominent results and recommendations. The research reached several results, the most notable of which are: The Jordanian constitution stipulated the separation of executive, legislative, and judicial powers, by placing the articles related to each power in a separate chapter from the other powers. They are organized in a way that ensures that the three powers do not interfere with each other. The aim of this is to prevent the concentration of power and to provide checks and balances. The Algerian, Egyptian, and Mauritanian constitutions all see that it is necessary to adopt the principle of separation of powers, and this was emphasized in the formulation of the constitutional texts, even though it was not applied on the ground to a large extent, as there is no complete separation of powers.