The purpose of study was to find the effect of hypovitaminosis D on concentration of parathyroid hormone (PTH) & its correlation with anthropometric measures in obese women residing in Lahore, Punjab Pakistan. A total of 128 women aging 20-50 years were selected from the outdoor patient’s department, Mayo Hospital Lahore. Non-obese women (n=25) were selected as control. This was a cross- sectional study and sampling was done according to random sampling technique. A written and informed consent of participation was taken from each woman before selecting as sample. Collection of blood sample was done to examine 25(OH)D and PTH by radioimmunoassay (RIA) and immunoradiometric assay (IRMA). Mean, standard deviation, student t-test and Pearson’s correlation coefficient were used for data analysis at 95% confidence interval and p-value <0.05. The average age of the sample and control group was 33.19±7.9 and 32.16±6.52 years, respectively. The mean 25(OH)D concentration in sample (14.91±6.16 ng/mL) verses control was (17.05±10.72; p<0.05). Serum PTH levels (26.12±17.94 vs.20.57±6.92 pg/mL) was approximately 5% higher in obese group as compared to control. Hypoparathyroidism was observed in 12.5% (sample) and 4% (control) population respectively. PTH shows negative correlation with weight (r=0.00011), height (r=-0.106), waist circumference (r=-0.147) and positive with BMI (r=0.05) and age (r=0.16). Vitamin D shows negative relation with BMI (r=-0.02), PTH (r=-0.015) and age (r=-0.14) and positive with weight (r=0.24), height (r=0.12), waist circumference (r=0.10) and hip circumstances (r=0.12). This data indicates frequent vitamin D inadequacy which is significantly altering the PTH level in female population.