1. MOHAMED ABOSHAMA - Lecturer, Removable Prosthodontic Department, Faculty of Dental Medicine, Al- Azhar University (Assiut),
Assiut Branch, Egypt.
2. MOHAMED. E. ALI - Lecturer, Microbiology and Immunology Department, Faculty of Pharmacy, Al- Azhar University (Assiut),
Assiut Branch, Egypt.
3. MAHMOUD EL-SAID AHMED ABD EL-AZIZ - Lecturer, Dental Biomaterials, Faculty of Dental Medicine, Al- Azhar University (Assiut), Assiut Branch, Egypt.
4. MAHMOUD AHMED MIZAR - Lecturer, Oral Medicine, Periodontology, Oral Diagnosis and Dental Radiology, Al-Azhar University
(Assiut), Assiut Branch, Egypt.
5. AHMED MOHAMED ABD ALRAHMAN - Lecturer, Oral Medicine, Periodontology, Oral Diagnosis and Dental Radiology, Al-Azhar University
(Assiut), Assiut Branch, Egypt.
6. SHARAF M.Y - Lecturer, Department Of Prosthodontics, Faculty Of Dentistry, Misr University Of Science And Technology,
6th October City, Egypt.
7. AHMED FATHEY ELHAGALI - Lecturer, Removable Prosthodontic Department, Faculty of Dental Medicine, Al-Azhar University (Assiut),
Assiut Branch, Egypt.
Purpose: The purpose of this in vitro study was to measure C. albicans' metabolic activity and biofilm adhesion on 3D printed acrylic resin. Material and methods: A total of 240 3D-printed acrylic resin specimens were constructed. A negative control group with 0 mg/mL of nicotine and 0 mg/mL of caffeine served as the test group for the ten subgroups (n=12), which were assessed for metabolic activity and biofilm development assessment. The remaining 9 subgroups had 8.00 mg/mL of nicotine as well as 9 distinct exponentially ascending caffeine concentrations at the following levels: 0, 0.25, 0.50, 1.00, 2.00, 4.00, 8.00, 16.00, and 32.00 mg/ml in each segment. Metabolic activity was assessed by using a 2,3-bis (2-methoxy-4-nitro-5-sulfophenyl)-2H-tetrazolium-carboxanilide (XTT). The dislodged biofilm underwent spiral plating at two dilutions (1:100 and 1:1000). 48 hours were spent incubating the plates at 37 C with 5% CO2. An automated colony-counting device (Protocol 3; Symbiosis) was used to count the yeast colonies and calculate the CFU/ml. Results: There was a statistically significant difference (p>0.05) between all groups and control group regarding the color changes. While regarding no of colonies (the CFU/ml) there was no significant difference between control group and ((0/8), (0.25/8) and (0.5/8)) while for remaining subgroups there was a significant difference (p>0.05). Conclusion: The presence of 8 mg/mL of nicotine alone increased the metabolic activity and biofilm formation of C. albicans. In the presence of 8 mg/mL of nicotine with different caffeine concentrations, overall, caffeine at higher concentrations (1.00, 2.00, 4.00, 8.00, 16.00, and 32.00mg/mL) inhibited biofilm formation of C. albicans on the specimen
Caffeine, Nicotine Metabolic Activity, Biofilm Adhesion, Candida Albicans, 3D Printed Acrylic Resin