Effective bio-medical waste management is crucial for preventing the spread of infections, protecting the environment, and ensuring the safety of healthcare workers and the community. This research paper aims to evaluate the knowledge, attitude, and practice of bio-medical waste management among healthcare workers in hospital settings. The study will employ a cross-sectional survey design, targeting healthcare workers from various departments in selected hospitals. A structured questionnaire will be used to assess their knowledge regarding bio-medical waste segregation, storage, transportation, and disposal, as well as their awareness of associated risks and regulations. The attitude of healthcare workers towards bio-medical waste management will be explored, including their perception of its importance, their motivation to comply with guidelines, and their willingness to adopt proper waste management practices. Factors influencing attitude, such as training programs, availability of resources, and organizational support, will be examined. The study will also assess the actual practice of bio-medical waste management among healthcare workers, including segregation practices, usage of appropriate containers, adherence to disposal guidelines, and utilization of personal protective equipment. Compliance with regulatory standards, such as color coding and signage, will also be evaluated. Data analysis will involve descriptive statistics, correlation analysis, and regression modeling to identify the factors associated with knowledge, attitude, and practice of bio-medical waste management among healthcare workers. The findings will inform the development of targeted interventions and training programs to improve waste management practices in healthcare settings.