Effective infection control practices are essential for minimizing the transmission of healthcare-associated infections (HAIs) and ensuring patient safety. This research paper aims to assess the knowledge of infection control practices among healthcare workers and compare it across different departments and experience levels in hospital settings. The study will utilize a cross-sectional design, targeting healthcare workers from various departments, including doctors, nurses, and support staff, in selected hospitals. A structured questionnaire will be used to assess their knowledge regarding standard precautions, hand hygiene, personal protective equipment (PPE) usage, safe injection practices, and environmental hygiene. The survey will also capture demographic information, years of experience, and previous infection control training received by the participants. Data analysis will involve descriptive statistics, including mean scores and percentages, to assess the overall knowledge level among healthcare workers. Furthermore, the knowledge scores will be compared across different departments and experience levels using appropriate statistical tests. The findings will provide insights into the gaps in knowledge regarding infection control practices among healthcare workers. This information can be utilized to develop targeted educational interventions and training programs to enhance their understanding and adherence to infection control guidelines.