The COVID-19 pandemic has placed significant psychological burdens on healthcare workers, leading to increased mental stress and burnout. This research paper aims to assess the mental stress experienced by healthcare workers during the pandemic and compare it across different healthcare settings and professional roles. The study will employ a cross-sectional design, targeting healthcare workers from various healthcare settings, including hospitals, clinics, and long-term care facilities. A standardized questionnaire, such as the Perceived Stress Scale (PSS), will be used to assess the levels of mental stress among participants. The survey will also collect demographic information, work-related factors, such as work hours and direct patient contact, and coping strategies employed by healthcare workers to mitigate stress. Data analysis will involve descriptive statistics to determine the overall levels of mental stress. Furthermore, the stress levels will be compared across different healthcare settings and professional roles using appropriate statistical tests. The findings will provide insights into the mental stress experienced by healthcare workers during the pandemic and shed light on the factors contributing to heightened stress levels. This information can be utilized to develop targeted interventions, support systems, and mental health programs to address the mental well-being of healthcare workers.