Manuscript Title:

ISOLATION, IDENTIFICATION AND TOXIN PRODUCTION FROM VAGINAL CLINICAL ISOLATES OF CANDIDA ALBICANS

Author:

KAWTHER H MHDEI, ZAHRA ABD AL-KARREM, ENAS RAHEEM AL-KIDSAWEY, ZAINAB SAJID MOHAMMED ​

DOI Number:

DOI:10.17605/OSF.IO/6NHQP

Published : 2021-09-10

About the author(s)

1. KAWTHER H MHDEI - Medical laboratory techniques.Hilla University College, Babylon, Iraq.
2. ZAHRA ABD AL-KARREM - Medical laboratory techniques .Hilla University College, Babylon, Iraq.
3. ENAS RAHEEM AL-KIDSAWEY - Medical laboratory techniques .Hilla University College, Babylon, Iraq.
4. ZAINAB SAJID MOHAMMED ​- Ahlulbait university/Iraq- Karballa / College of Pharmacy.

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Abstract

Thirty two isolates of candida albican were sequestered from female patients with vaginal ulcer ( lying in marjan hospital hillia city) .The vaginal swap were cultured on sabouraud dextrose broth and agar plate espectively . for differential identification of C.albican ,Hi chrome candida agar was used . Gram staining used to smear of vaginal scrapings . suspected isolates of C.albican then immunized on corn meal agar mediocre for chlamydospore, formation , for germ tube formation the isolates of C.albican was cultured in human serum .Carbohydrate assimilation and fe rmentation tests also used for further identification . four identification s trains of C.albican namely ( CA1, CA2, CA3, CA4) were selected according to their antagonistic activity against standard strain of bacteria E.coli NCTC5933 and staphylococcus aureus NCTC6571 ; To produced extracellular toxin of glycoprotein nature in solid media and fermentation liquid media . The toxin which produced (secondary metabolites) don’t effecting the producers strains of C.albican (CA1,CA2,CA3,CA4) ,but the produced toxin was inhibited the growth of standard strain of candida albicans ATcc8201( sensitive ). Simple method used for toxin extraction by using ethanol 90%.And the extracted toxin from free cells supernatant . cytotoxicity of toxin against human blood cells were 6 ppm . also the stability of producing toxin was determined.


Keywords

candida albicans , germ tube ,conventional technigues ,mycotoxin, vaginal swaps.