Manuscript Title:

EFFECT OF DIPLAZIUM ESCULENTUM ON CHOLINESTERASE, PARAOXONASE-1 AND MALONDIALDEHID ACTIVITIES ON VEGETABLE FARMERS USING PESTICIDES

Author:

HABIBI, SURYANI ASAD, ARMYN NURDIN, AGUSSALIM BUKHARI, M.FURQAAN NAIEM, NILDAWATI, MUHAMMAD AZRUL SYAMSUL

DOI Number:

DOI:10.17605/OSF.IO/Q6MS3

Published : 2022-06-10

About the author(s)

1. HABIBI - Department of Medical Science, Faculty Of Medicine, Hasanuddin University, Makassar, Indonesia & Department of Public Health, Faculty Of Medical And Health Sciences, Universitas Islam Negeri Alauddin, Makassar, Indonesia.
2. SURYANI ASAD - Department of Nutritional Science, Faculty Of Medicine, Hasanuddin University, Makassar, Indonesia.
3. ARMYN NURDIN - Department of Community Medicine, Faculty Of Medicine, Hasanuddin University, Makassar, Indonesia.
4. AGUSSALIM BUKHARI - Department of Nutritional Science, Faculty Of Medicine, Hasanuddin University, Makassar, Indonesia.
5. M.FURQAAN NAIEM - Department of Occupational Health And Safety, Faculty Of Public Health, Hasanuddin University, Makassar, Indonesia.
6. NILDAWATI - Department of Public Health, Faculty Of Medical And Health Sciences, Universitas Islam Negeri Alauddin, Makassar, Indonesia.
7. MUHAMMAD AZRUL SYAMSUL - Department of Industrial Hygiene, Occupational Health And Safety, Sekolah Tinggi Ilmu Kesehatan Makassar, Makassar, Indonesia.

Full Text : PDF

Abstract

BACKGROUND: The use of pesticides in the world is currently increasing and even uncontrolled and poses a risk of pesticide poisoning for farmers. Diplazium esculentum is a biodiversity in Indonesia and contains active compounds include steroids, triterpenoids, phenols, flavones and high antioxidant potential, which contains flavonoid compounds that have the potential to reduce pesticide poisoning. AIM: This study aims to determine the effect of Diplazium esculentum on enzyme cholinesterase activity, paraoxonase-1 (PON1) activity and malondialdehyde (MDA) activity in farmers exposed to pesticides.

METHODS: This study uses a Randomized Controlled Trial (RCT) experimental design, in which the researcher allocates research samples to the experimental group and the control group randomly based on the criteria determined by the researcher, double-blind. Examination of Cholinesterase Activity, Paraoxonase-1 (PON1) and Malondialdehyde (MDA) Test with Enzyme-linked Immunosorbent Assay (ELISA) method. The population in this study were all pesticide-using vegetable farmers in Kanreapia Village, Tombolopao District, Gowa Regency, which consisted of 375 people. While the number of samples was 42 people (21 people in the intervention group and 21 people in the control group).

RESULTS: Based on the paired t test, the value in the intervention group was p = 0.004 and the control p = 0.032 and the results of the unpaired t test obtained a significance value of p = 0.047 which means there is an effect of giving Diplazium esculentum activity cholinesterase The results of the Wilcoxon paraoxonase1 showed that the value in the intervention group was p=0.005 and the control group p=0.520.test Mann Whitney U obtained a significance value of p = 0.043 which means that there is an effect of giving Diplazium esculentum levels paraoxonase-1 Whereas Wilcoxon malondialdehyde (MDA) The value in the intervention group was p=0.424 and the control p=0.375.test Mann Whitney U showed a significance value of p=0.242, which means that there was no significant effect of giving Diplazium esculentum levels malondialdehyde in pesticide-using vegetable farmers.

CONCLUSIONS: There is an effect of Diplazium esculentum on enzyme cholinesterase, and paraoxonase-1 (PON1) activity, and there is no effect of Diplazium esculentum on malondialdehyde (MDA) in farmers exposed to pesticides.


Keywords

Diplazium esculentum; enzyme cholinesterase, paraoxonase-1 (PON1) activity; malondialdehyde (MDA) in farmers exposed to pesticides.