1. SARITHA PENDYALA - Department of Biotechnology, Mahatma Gandhi University, Nalgonda 508001, Telangana, India.
2. MADHURI KULKARNI - Department of Biotechnology, Mahatma Gandhi University, Nalgonda 508001, Telangana, India.
Biosurfactant (BS) production by Bacillus vallismortis RMS25 is investigated using keratin rich wastes as medium components. Biosurfactant production was observed to be higher under submerged fermentation conditions (1.12 g/L) when chicken feathers were used as medium ingredient. Plackett–Burman statistical design (PBD) and response surface methodology (RSM) are used to screen different keratinous materials and to optimize BS production. With a correlation coefficient (R2 ) of 97%, a maximum BS production of 1124 mgL-1 (3-fold increase) was validated under optimized conditions (chicken feather 4 %, glucose 4 %, inoculum size 2 %, and 48-hour incubation period). The findings point to an environmentally friendly method for biodegrading chicken feather waste and the use of currently studied substrate minimizes the production cost significantly.
Biosurfactant, Plackett–Burman design, correlation coefficient, Response surface methodology, chicken feather.