Objective: to determine the knowledge attitude and practices of dental professional against endemic contagious diseases. Setting and Time: The study conducted at tertiary care hospital in Karachi, from June 2022 to Jul 2022. Sampling: Non-probability convenient sampling Method: A sample of 300 male and female dentists, including final year students, house officers, P.Gs, and specialists, was surveyed in this study. Data was collected from mid-June 2022 to the end of July 2022 through a 39-question closed-ended questionnaire. The study's objective was to analyse the responses using SPSS in order to comprehend the perceptions and practices of dental professionals in various institutions. Results: 300 participants took part in the research in which males were 65(21.7%) in number and females were 234(78%). Musculoskeletal disorder was the main hazard which affects them with a prevalence rate of 59%. Conclusion: This study provides a comprehensive exploration of participant demographics and health-related factors, particularly focusing on Hepatitis B and HIV. The diverse gender representation and educational backgrounds underscore the study's broad scope. Key findings reveal significant associations in variables related to vaccination, needle pricks, and responses to Hb Positive blood exposure. These insights contribute to a nuanced understanding of both demographic characteristics and crucial health considerations among the surveyed population.