The purpose of this study was to determine the relationship between pesticide exposure and circulation levels of male reproductive hormones in agricultural community. To assess variations in serum testosterone level, eighty (80) male spray workers/farmers were participated and exposed to the parathyroid lambdacyhalothrin. A comprehensive questionnaire was used to collect extensive information about recent, collective lifetime use of pesticides and other agricultural- related activities. Participants' exposure to pesticide, protective tools, and health status were also assessed over the study period. Blood samples were sought before and after applications of pesticides to evaluate the serum testosterone levels. Results were assessed statistically. When compared to their pre-exposure levels 5.77±0.98ng/ml, it was recorded that sixty (60%) of the spray workers had significantly lower (P < 0.05) serum testosterone level 4.77±0.28 ng. /ml. Out of these 60%, (21%) spray workers had highly significant (P < 0.01) declined, serum testosterone level (2.79%±0.40ng/ml) was below normal. Dizziness, headaches, joint pain, nausea, and allergies to the skin and eyes were noted as clinical symptoms linked to the pesticide exposure. It has been shown that the pesticide lambda cyhalothrin may disrupt the hypothalamic-pituitary- testicular axis, which can reduce serum testosterone production and potentially harm spray workers' reproductive systems.