Knowledge, perception, and preventive measures with regard to COVID-19 among the university student are of immense importance and are critical for the community prevention/ control of this pandemic. Aim to assess the perception of university students in Palestine regarding preventive measures during COVID-19 pandemic. Research design: A cross-sectional survey consisted of 2094 university students. Setting: the study was conducted at Modern University College, An-Najah National University, Palestine Polytechnic University, Al-Quds Open University, Ibn Sina College of Health Sciences and Arab American of University /West Bank / Palestine. The data collected was from the participant university students and started in first October 2020 to end of December 2020. Subjects: A convenience sample with different Bachelor degree programs. Tools: A self-designed questionnaire was used for data collection and included the following parts. 1: demographic particulars of the university students, II. University students Knowledge are regarding Coronavirus, isolation for patient with Covid-19 at home, also their knowledge regarding following for preventive measures outside home from [15; [16], III: preventive measure as practices were assessed for university students outside home Part IV: assessed University student perception toward Covid-19. Results: the current study reveals that 80.2% of the studied sample of students had a satisfactory total knowledge regarding preventive measures outside home, 91% of them had a satisfactory total knowledge regarding preventive measures inside home among patient with Covid-19, and 95.5% of them had a satisfactory total knowledge regarding Covid-19 disease. There was a statistically highly significant relation between total knowledge and total practices outside home (p-value<0.001), and statistically significant relation between total knowledge and total perception (p-value0.005).Conclusion: based on the current study, students were observed to have substantial knowledge, practices for preventive measures, and a positive perception toward COVID-19panademic . Recommendation: It is the responsibility of the state to take all preventive measures for the safety of the community and public. There is an urgent need for research to address burden of the Covid-19 pandemic on college students.